Identification of Mesorhizobium loti Genes Relevant to Symbiosis by Using Signature-Tagged Mutants
نویسندگان
چکیده
منابع مشابه
Construction of Signature-tagged Mutant Library in Mesorhizobium loti as a Powerful Tool for Functional Genomics
Rhizobia are nitrogen-fixing soil bacteria that establish endosymbiosis with some leguminous plants. The completion of several rhizobial genome sequences provides opportunities for genome-wide functional studies of the physiological roles of many rhizobial genes. In order to carry out genome-wide phenotypic screenings, we have constructed a large mutant library of the nitrogen-fixing symbiotic ...
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In the model leguminous plant Lotus japonicus, the reception of a symbiotic signal called Nod factor (NF), which is secreted by the symbiont bacterium Mesorhizobium loti, induces wavy shaped root hairs. This is called root hair deformation. To dissect the root hair deformation process, we studied symbiosis- deficient mutants of L. japonicus, castor, nup85, ccamk and nsp2. The CASTOR, NUP85, and...
متن کاملComparative sequence analysis of the symbiosis island of Mesorhizobium loti strain R7A.
The Mesorhizobium loti strain R7A symbiosis island is a 502-kb chromosomally integrated element which transfers to nonsymbiotic mesorhizobia in the environment, converting them to Lotus symbionts. It integrates into a phenylalanine tRNA gene in a process mediated by a P4-type integrase encoded at the left end of the element. We have determined the nucleotide sequence of the island and compared ...
متن کاملExpression islands clustered on the symbiosis island of the Mesorhizobium loti genome.
Rhizobia are symbiotic nitrogen-fixing soil bacteria that are associated with host legumes. The establishment of rhizobial symbiosis requires signal exchanges between partners in microaerobic environments that result in mutualism for the two partners. We developed a macroarray for Mesorhizobium loti MAFF303099, a microsymbiont of the model legume Lotus japonicus, and monitored the transcription...
متن کاملCommonalities and Differences among Symbiosis Islands of Three Mesorhizobium loti Strains
To shed light on the breadth of the host range of Mesorhizobium loti strain NZP2037, we determined the sequence of the NZP2037 symbiosis island and compared it with those of strain MAFF303099 and R7A islands. The determined 533 kb sequence of NZP2037 symbiosis island, on which 504 genes were predicted, implied its integration into a phenylalanine-tRNA gene and subsequent genome rearrangement. C...
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ژورنال
عنوان ژورنال: Microbes and Environments
سال: 2011
ISSN: 1342-6311,1347-4405
DOI: 10.1264/jsme2.me10213